High performance liquid chromatography in pharmaceutical analyses

High Performance Liquid Chromatography (HPLC) in the pharmaceutical analysis

Introduction Drug manufacturing control requires high level and intensive analytical and chemical support of all stages to ensure the drug's quality and safety. The pharmacopeia constitutes a collection of recommended procedures for analysis and specifications for the determination of pharmaceutical substances, excipients, and dosage forms that is intended to serve as source material for refere...

High-Performance Liquid Chromatography Determination of Methotrexate in Plasma

This article describes a simple and fast high-performance liquid chromatography method for the determination of methotrexate (MTX) in plasma. Samples were collocted from children receiving high-dose MTX at shafa Hospital (Ahvaz University of Medical sciences, Ahvaz, iran) at various times after the end of eachinfusion. Plasma was deproteinized with trichloroacetic acid and the supernatant was i...

High-Performance Liquid Chromatography Determination of Methotrexate in Plasma

This article describes a simple and fast high-performance liquid chromatography method for the determination of methotrexate (MTX) in plasma. Samples were collocted from children receiving high-dose MTX at shafa Hospital (Ahvaz University of Medical sciences, Ahvaz, iran) at various times after the end of eachinfusion. Plasma was deproteinized with trichloroacetic acid and the supernatant was i...

High Performance Liquid Chromatography Determination of Sertraline in Human

       An accurate and sensitive reversed-phase high-performance liquid chromatograph-ic method for determination of sertraline in human serum is described using 4-chloro-7-nitrobenzofurazan as pre-column derivatization agent. The drug and an internal standard (azithrimycin) were extracted from serum using a mixture of diethyl ether-chloroform and subjected to the pre-column derivatization with...

development and validation of high performance liquid chromatography for determination of nystatin in pharmaceutical formulations